Biomedical research in cancer focuses nowadays on tridimensional cultures of cell aggregates, as they create a replica of cancer's development similar to the development in the human body. This methodology has been published at the virtual platform https://www.promocell.com/blog/a-home-for-cancer-stem-cells-3d-tumorspheres/ Promocell, based on the Dr. Matilde Lleonart research, head of the group http://en.vhir.org/portal1/grup-presentacio.asp?s=recerca&contentid=186740&idrefer=186741 Biomedical Research in Cancer Stem Cells at the Oncology Unit of Vall d'Hebron Research Institute (VHIR).Today, biomedical research characterizes cancer by creating tridimensional cell cultures, named tumorspheres. They act as tumors in the human body (in the structural, functional and physiological levels), and let focus on the cancer stem cells and their interactions with the surrounding cells and microenvironment, thus, cells are never alone, but always wrapped by other cells and an extracellular matrix that keeps them alive, creating tissues and tridimensional organs as we know them.Among this complexity, the cancer stem cells are the main goal of the researchers to improve cancer's therapy, as they are probably the main cause of metastasis and cancer recurrence after treatments by chemotherapy and radiotherapy, especially in brain cancers, breast cancers, colon cancers, liver cancers and lung cancers.By this means, it is expected that by deleting cancer stem cells, an ensured cure of cancer can be achieved. On the other hand, as Dr. Matilde E. Lleonart explains "no specific markers have been found for these cancer stem cells, forcing researchers to use general traits. We want to identify unique traits that could improve the characterization of the cancer stem cells".Replicating cancer stem cells from the human body to the laboratory: the tumorspheresSince the 90s, 3D cell cultures are the more representative of the in vivo environment, due to its replication capacity into tridimensional and complex environments, offering a unique comprehension of the interactions between the host and the tumor, or the tumor and the microenvironment.This kind of culture brings a better understanding of cancer's cell complexities, and is reached by using culturing media as PromoCell 3D Tumorsphere Medium XF and PromoCell Primary Cancer Culture System."Our research creates in vitro models that resemble the in vivo situation as much as possible. By this means, we can study the phenotypic and molecular changes in cancer cells under several conditions, grow tumorspheres from a single cancer stem cell, and find new molecular markers, as well as isolate cancer stem cells from several types of cancer as breast, lung, head and neck", details Dr. Matilde Lleonart.Getting closer to the personalized medicine:This type of research can create therapeutic simulations, personalized per patient, by culturing from the patient's samples tumorspheres to experiment in the lab with the possible treatments against the detected tumor, and select the most effective for the patient.