Pamela Dominguez Baez I have more than 3 years of experience in the research sector. I am a person who is passionate about his work, I like to work in a team and generate a good work environment, I am orderly, with initiative, communicative, rigorous, meticulous, curious, decisive, flexible, proactive, innovative, constant, effective, respectful, I work I do well under pressure and I adapt quickly to changes and to the job. I like and I am passionate about my work, I want to improve in everything. In my work at the VHIR in the Vall d'Hebrón Nephrology and Transplantation group, I carry out tasks such as registration, management, processing and analysis of samples of animal and human origin, such as urine, serum and plasma, tissues of kidney, heart, lung, pancreas among others through the use of techniques such as RNA extraction and quantification, PCR, qPCR performing histology work, staining, cell cultures, WB, ELISA, protein quantification, buffer preparation, stock control , order management and maintaining order in the laboratory among other functions of the position, in addition to working as an animal experimentation technician. Institutions of which they are part Research technician Bioengineering, Cell Therapy and Surgery in Congenital Malformations Vall Hebron Institut de Recerca Email Pamela Dominguez Baez Email Institutions of which they are part Research technician Bioengineering, Cell Therapy and Surgery in Congenital Malformations Vall Hebron Institut de Recerca I have more than 3 years of experience in the research sector. I am a person who is passionate about his work, I like to work in a team and generate a good work environment, I am orderly, with initiative, communicative, rigorous, meticulous, curious, decisive, flexible, proactive, innovative, constant, effective, respectful, I work I do well under pressure and I adapt quickly to changes and to the job. I like and I am passionate about my work, I want to improve in everything. In my work at the VHIR in the Vall d'Hebrón Nephrology and Transplantation group, I carry out tasks such as registration, management, processing and analysis of samples of animal and human origin, such as urine, serum and plasma, tissues of kidney, heart, lung, pancreas among others through the use of techniques such as RNA extraction and quantification, PCR, qPCR performing histology work, staining, cell cultures, WB, ELISA, protein quantification, buffer preparation, stock control , order management and maintaining order in the laboratory among other functions of the position, in addition to working as an animal experimentation technician.
ACADEMIC TRAINING Higher Technician FP Degree in Clinical Analysis and Diagnosis Laboratory, Colegio Aloya.Vigo.2011-2013. Intermediate FP Title in Nursing Assistant (Aux. Clinic). Mendiño School. Vigo. 2009-2010. Graduate Certificate in E.S.O. in science. I.E.S. Polytechnic of Vigo.2006-2007. Courses: Training course for experimenters in laboratory animal science category B. Research and Science Institute of Castilla y León, University of Salamanca.(40h), with practices on animals. February 2014. First Aid and CPR Course (50h). at ESPS.Vigo.2010. Occupational Risk Prevention Course in Natural Therapies (50h) at ESPS.Vigo.2010. Functional Bandages and Taping Course (50h) at ESPS.Vigo.2010. Social Skills Course (50h), Aloya, Vigo. 2013. Professional Ethics Course (50h), Aloya, Vigo. 2013. Food Handler Card, postal academy 10, Ourense 2013. WORK EXPERIENCE Laboratory Technician for diagnosis and clinical analysis and Animal Experimentation Technique in the Nephrology and Transplantation research group. Routine laboratory work following GLP and SOPs, such as the processing and analysis of biological samples from animals and humans using WB, PCR, RT-PCR techniques, urine concentration, protein quantification, tissue protein and protein extraction. membrane, cell culture protein, RNA extraction, serum activity assays, cell cultures, immunohistochemistry, immunofluorescence, tissue staining such as hematoxylin, eosin, PAS, fast green, microscopic observation and identification, pathological anatomy techniques such as inclusion in paraffin, tissue fixation, sectioning tissues with the microtome, preparation of buffers and reagents, equipment calibration, stock control, placing orders, preparation of PNTs, among others of the position. Carrying out experimental studies in mice, measurement of glomerular filtration rate, surgery (nephrectomy), IV, IM, IP, SB, O, PC administration, blood glucose determination, blood pressure recording, euthanasia, among others. VHIR.2019-2022. Stabular technician-caretaker, performance of routine stable procedures with rodents following GLP and PNTs, weighing, housing, control of clinical signs, cage changes, administration via O, SB, IP, IV, IM, euthanasia, temperature control and humidity deblas rooms, elaboration of PNTs, among others. Vivotecnia Research for Vall d'Hebrón, 4 months, 2019.Barcelona. Hospital Nursing Assistant. Functions performed: taking vital signs, preparing diets, hygiene, preparing the patient for surgery, among others. Hospital Fremap. 2 months, 2019.Barcelona. Animal Research Laboratory Technician. Functions performed: administration of substances in animals, dogs, rats and rabbits, routine stable work, sample collection, control of clinical signs, euthanasia, among others, always under GLP and PNTs. 1 year and 6 months, 2018, Envigo CRS Laboratories. Sant. Perpetua of Moguda. Nursing Assistant Hospital Josep Trueta, February to June 2015, Gerona. Internship as a Laboratory Technician at the Meixueiro Hospital, 3 months passing through the areas of: hematology, proteins, urine, hormones, coagulation, emergencies, immunology, biochemistry, microbiology and sample registration. Performing smears, stains, ELISA tests, PCR, HPLC, blood counts, proteinograms, DNA analysis, RNA, PH measurement, osmosis of samples, preparation of culture media, seeding, change of reagents, microscopic identification, among others. 2013,Vigo. Piñeiro and Pardo Dental Clinic. 2012,Vigo. Doral Residences Day Center, 8 months 2011, Vigo. Auxiliary practices. of Nursing in Assisted Residence and Day Center, Doral Residencias, 2010, Vigo.
Projects Nefrologia i Trasplantament Renal IP: Oriol Bestard Matamoros Collaborators: Marina Lopez Martinez, Francesc Moreso Mateos, Natalia Ramos Terrades, Maria Jose Soler Romeo, Joana Sellarés Roig, Pamela Dominguez Baez, Vergara Arana, Ander, Irene Agraz Pamplona, Sheila Bermejo Garcia, Mariona Juvé Torres, Irina Betsabe Torres Rodriguez, Alba Torija Recasens, Concepció Jacobs Cachá, Elena Isabel Crespo Gimeno, Laura Donadeu Casassas, Carmen Llorens Cebriá, Nuria Bolaños Peruga, Manel Perelló Carrascosa, Maria Antonia Emilia Meneghini Funding agency: Agència Gestió Ajuts Universitaris i de Recerca Funding: 40000 Reference: 2021 SGR 00883 Duration: 01/01/2022 - 30/06/2025 Desentrañando las vías protectoras de los SGLT2i y GLP-1RA en la enfermedad renal diabética: papel de las proteínas adaptadoras MAP17 y D-AKAP-2 en ratones db/db y organoides renales humanos. IP: Maria Jose Soler Romeo Collaborators: Mario Alonso Narvaez Barros, Pamela Dominguez Baez, Vergara Arana, Ander, Sheila Bermejo Garcia, Alejandra Planas Vilaseca, Concepció Jacobs Cachá, Juan Carlos León Román Funding agency: Instituto de Salud Carlos III Funding: 122210 Reference: PI21/01292 Duration: 01/01/2022 - 31/12/2024
