In DBA/2 J mice, mutations in two genes (GpnmbR150X and Tyrp1b) lead to iris disease with features of human iris atrophy and pigment dispersion syndrome. This strain of mice is used as an experimental model of POAG (primary open-angle glaucoma), as they develop a chronic progressive increase in IOP that causes retinal ganglion cell death. The increase in IOP in these animals begins at 10 months of age, and the pressure remains elevated until death. These animal models are very useful in elucidating the glaucoma pathogenesis and to identify potential therapeutic targets

Measurement of intraocular pressure (IOP), reading is obtained from the central cornea of each eye. is measured with a rebound tonometer (Tonolab, Icare, Espoo, Finland) specific adapted by mice or rat

Pterional craniotomy and subsequent surgical occlusion of both branches of the middle cerebral artery

Human PBMC.s will be inoculated intraperitoneally.

Model generated by mouse kidney androgen-regulated protein (KAP) cDNA cloned into pKAP2 vector, microinjected into 1-cell–fertilized B6SJL F2 embryos.

1 cycle of 2.5% DSS in drinking water ad libitum for 5 days, followed by 7 days of regular water to mimic the acute phase of the disease

Rats are administered omeprazole (50 mg/Kg/dia) for 3 days prior to intestinal decontamination in order to facilitate re-colonization with transplanted stool. To achieve intestinal emptying, rats are maintained isolated in fast grills with free access to water, and two oral doses of CitraFleet (sodium picosulfate, 0.16mg/mL and magnesium oxide 51.2mg/mL) of 1 ml and 2 ml are administered 24h and 12h, respectively, before the transplant (accompanied by 2 ml of water each). Re-colonization is performed by a single oral gavage with 100 mg of the corresponding faecal pool diluted in 2 ml of sterile PBS.

IBS model is based on generating psychological stress in animals. On the one hand, there is competition for space as 8 animals / cage are placed on an area of ​​1000 cm2. In addition, there is competition for access to food and drink, the floor contains more shavings than usual, which makes it difficult to move. In addition, in order to generate additional stress that accentuates social stress, a "Water Avoidance Stress (WAS)" test will be performed on days 3, 7, 10 and 15 of the study. This model of stress is widely described and used because it mimics the environmental stress that human beings suffer . This model consists of placing the animal - for 30 minutes - on a platform (8 x 6 cm) in a plastic container filled with warm water (25ºC) up to 1 cm below the platform. For healthy animals, the same test is performed but without water.At the end of the study day (day 15), different variables will be analyzed, specifically intestinal barrier function (ussing chambers, gene expression and immunohistochemistry studies) and abdominal hypersensitivity.

Transgenic mice to study GNB1 Encephalopathy

Choroidal vessel growth into the retina after laser-induced Bruch's membrane break.