Intraperitoneal injection of ID8 ovarian cancer cells

Cancer cells will be injected subcutaneously since early tumor formation

Subcutaneous implantation of tumor cells for efficacy, biodistribution, PK/PD and toxicity assays

Subcutaneous injection of cancer cells: pancreatic (BxPC3, MIA PaCa-2), lung (A549), colon (SW480), glioblastom ( U251, T98G)

Tumor cells will be inoculated subcutaneous in the caudal dorsal flank.

Primary tumor generated from cell lines.

The coronary artery will be identified and blocked using a 4/0 silk, placed 1 mm distal to the tip of the left atrium. All animals will undergo 60 minutes of coronary occlusion followed by 24 or 21 days of reperfusion. Function is assessed by echocardiography.

Model generated by administration of monocrotaline pyrrole and aorto-caval shunt surgery

The RCS rat strain provides a model of inherited outer retinal degenerative diseases. At 4 weeks of age, the RCS rat retina appears normal. However, by 8 weeks of age, and under low light conditions, RCS rats have impaired retinal function and disorganized and thinned retina. Standard lighting conditions accelerated the degenerative process while low lighting conditions slow the process thereby enabling a broader window for therapeutic intervention. The RCS rat strain is a good model for evaluating efficacy of gene based therapies and/or retinal prosthesis devices. July 2019. Volume 60, Issue 9. OPEN ACCESS. ARVO Annual Meeting Abstract. July 2019. Characterization of the Royal College of Surgeon’s Rat Model Endpoints for Use in Preclinical Ophthalmic Studies Justin Prater; Vicky Peele; David Culp; Brian C Gilger. Availability: Cryopreserved

With this model, cirrhosis is caused by biliary poisoning. At 2 weeks the model has mild portal hypertension. The appearance of the animal at 28 days is characterized by jaundice, dark urine and, in 20% of the rats, by some degree of ascites. The model will stop at 4 weeks, at which time the rat has been shown to have established cirrhosis.