The DKO rd8 is obtained by crossing the C57BL/6NJ strain (homozygous for the Crb1rd8 mutation, the B6.129S4-*Ccl2tm1Rol*/J strain (homozygous for the Ccl2tm1Rol (Ccl2-/-) mutation), and the B6. 129P-*Cx3cr1tm1Litt*/J strain (homozygous for the Cx3cr1tm1Litt (Cx3cr1-/-) mutation). Availability: Cryopreserved

Model generated by Implantation of subcutaneous angiotensin II release pump

Model generated by Implantation of subcutaneous isoproterenol release pump

This model exhibits servere obesity and altered glucose homeostasis (glucose intolerance and insulin resistance) after being fed with a high fat diet (45% Kcal from fat) or very high fat diet (60 % kcal from fat).

The low-dose streptozotocin model has been widely used for years in numerous publications with excellent results. The main advantage of drug delivery-based models is that the degree of β-cell disruption can be regulated according to the dose of toxin administered. This diabetic rat constitutes a good model because it generates vascular dysfunction and retinal neurodegeneration similar to human pathogenesis and allows obtaining sufficient amounts of retinal RNA or protein for experimental procedures. 1 dose of 65 mg/kg of STZ solution intraperitoneally. A week after stabilization, weight and blood glucose are measured. If the injected rats have a blood glucose level of > 250 mg/dl, they are considered diabetic.

Dexamethasone induces intraocular hypertension and a degeneration of the optic nerve

Model generated by intramuscular injection of cyclosporine (CsA)

Ablation of Cx43 expression is achieved by 4-hydroxytamoxifen (4-OHT) treatment in adult mice intraperitoneally injected once a day, for 5 consecutive days, with 3 mg of 4-OHT.

Rats receive the first week a dose of 250mg/Kg IP of TAA twice a week, the following 7 weeks they receive a dose of 200mg/Kg IP twice a week, being a total of 8 weeks and 16 injections After 8 weeks of treatment at a dose of 250-200mg/kg, twice a week, all animals will develop histological features of cirrhosis

With the administration of CCl4 we produce an acute hepatitis, and with its continued administration we generate a chronic liver injury that leads to the development of cirrhosis. To speed up the model, phenobarbital is administered to water (0.3 g/L) one week before starting the hepatotoxic exposure and until the end of the experiment We will consider that the animal has developed cirrhosis with portal hypertension after 12 weeks of inhalation.