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Nanopartícules Farmacocinètiques

El grup de Nanopartícules Farmacocinètiques del Vall d’Hebron Institut de Recerca es centra en la síntesi, caracterització i aplicacions de nanopartícules inorgàniques en el camp de la medicina. En controlar el nucli inorgànic (mida, estructura i forma) i la unió de molècules a la seva superfície, dissenyem nanopartícules que interactuen amb els sistemes biològics d'una manera específica. També posem especial èmfasi en la seguretat, viabilitat, ampliació, aplicabilitat i altres aspectes del disseny de nanopartícules per a la medicina.

El nostre grup està dirigit pel professor Víctor Puntes, qui té una Càtedra d'Investigació ICREA. Atès que una gran part del nostre treball exigeix una experiència altament multidisciplinària, mantenim col·laboracions amb físics, biòlegs, immunòlegs, oncòlegs i altres, d'Europa, la Xina i els EUA. El treball del grup ha donat lloc a diverses patents i a la creació d’empreses derivades (spin-off) com Nanotargeting i Applied nanoparticles.

Equip

Comenge Farré, Joan

Comenge Farré, Joan

Investigador/a postdoctoral
Nanopartícules farmacocinètiques
Llegir més
Franco Puntes, Victor

Franco Puntes, Victor

Cap de grup
Nanopartícules farmacocinètiques
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Montaña Ernst, Lena Nerea

Montaña Ernst, Lena Nerea

Investigador/a predoctoral
Nanopartícules farmacocinètiques
Llegir més
Ramis Garcia, Joana

Ramis Garcia, Joana

Investigador/a predoctoral
Nanopartícules farmacocinètiques
Llegir més
Salazar Montoya, Vivian Angelica

Salazar Montoya, Vivian Angelica

Investigador/a postdoctoral
Nanopartícules farmacocinètiques
Llegir més
Comenge Farré, Joan

Comenge Farré, Joan

Investigador/a postdoctoral
Nanopartícules farmacocinètiques
Llegir més
Franco Puntes, Victor

Franco Puntes, Victor

Cap de grup
Nanopartícules farmacocinètiques
Llegir més
Montaña Ernst, Lena Nerea

Montaña Ernst, Lena Nerea

Investigador/a predoctoral
Nanopartícules farmacocinètiques
Llegir més
Ramis Garcia, Joana

Ramis Garcia, Joana

Investigador/a predoctoral
Nanopartícules farmacocinètiques
Llegir més
Salazar Montoya, Vivian Angelica

Salazar Montoya, Vivian Angelica

Investigador/a postdoctoral
Nanopartícules farmacocinètiques
Llegir més

Línies de recerca

Design of nanoparticles for useful applications

Getting advantages from the accumulated experience, I will study the most problematic points related to the growth of multi-component materials and hollow structures. We are preparing complex multifunctional environment responsive NPs comprising different families: i) metallic, ii) metal oxide, iii) semiconductor and iv) oxides of semiconductor. Beyond synthesis, I will focus on the precise characterization of the obtained NPs in terms of their reactivity and physico-chemical properties to precisely correlate morphology with activity. The synthesis of advanced NPs will be carry out, primarily, following seeding-growth approaches or by combining the simultaneous or sequential injection of precursor solutions, surfactant mixtures. The temperature of the solution will be adjusted in order to kinetically control the formation of the NPs and reaction times will be controlled to induce digestion processes and re-crystallizations/controlled sintering when required.  I will use mixtures of surfactants or reducing agents and coordinating complexes under controlled atmospheres, intending to independently modify the solubility of the monomer, the nucleation radii, the growing rate and the morphology of the growing structure (by stabilizing different crystals phases and therefore lowering their energy and accelerating selected competing reactions). Hollow metal structures will be synthesized following a multi-step procedure. In the first step, Ag nanocubes are synthesized by known techniques (such as polyol synthesis). Polymetallic hollow NPs with very different morphology  and composition, will be obtained by the simultaneous or sequential action of galvanic replacement and the Kirkendall effect in order to control the number and morphology of void spaces inside a single NP, and the chemical transformation of NPs such as cation release/exchange. This allows the production of, among other, monodisperse single- and double-walled nanoboxes or noble metal fullerenes, in high yields and scalable synthesis.


a. APPLICATION ON MEDICINE.

Nanotechnology’s ability to shape matter at the scale of molecules is opening the door to a new generation of diagnostics, imaging agents and drugs/therapies for detecting and treating disease. But perhaps more important,  it is allowing to combine a series of advances, creating nanosized particles that may for example contain drugs designed to kill damaged cells together with targeting compounds designed to home-in on malignancies, and imaging agents designed to light up even the earliest stage of disease. For example NPs are perfect candidates to be used in anticancer therapy since they showed passive accumulation in solid tumours due to the Enhanced Permeation and Retention effect (EPR). Is in this context that nanotechnology emerges as a “disruptive technology” with a great potential to contribute to improve treatment by generating new diagnostic and therapeutic products. Its fields of action can be classified in diagnosis, imaging, drug delivery, hyperthermia, theranostics, the simultaneous diagnosis and therapy, and therapy monitoring.


It has been acknowledged that one of the most promising societal impacts of nanotechnology is in the area of nanomedicine. Personalized health care, rational drug design and targeted drug delivery are some of the proposed benefits of a nanomedicine-based approach to therapy. On this subject, my lines of work are: i) Nanooncology, the use of nanoparticles for diagnosis and treatment of cancer. Here I am developing NP-Biomolecule (as AuNP-Antibody) for detection (as lung cancer in breath or circulating cancer cells) and AuNP and Fe3O4 NP for diagnosis/imaging. I continue my work carrying antitumoral drugs with AuNPs (as cisplatin, oxaliplatin, carboplatin, doxorubicin, and sorafenib) and their use as radiotherapy enhancers or hyperthermia agents. ii) Immunology. Significantly important is the interaction of NPs with the immune system. Because one cannot put stuff inside the body without asking the immune system for permission, I am studying the effect of NP on antigen presentation, to then avoid immune detection (for a drug delivery vehicle) or design prophylactic and therapeutic vaccines (for molecular scaffolds). This includes as much AuNPs decorated with antigens as redox active anti-inflammatory CeO2 NPs. iii) Antimicrobial NPs. The emergence of antibiotic resistant strains of common pathogens is a major threat to health and is already putting tremendous pressure on health services worldwide. It has been shown that positive (cationic) nanoparticles show toxic effects to prokaryote, as defensines do. Also, silver, iron or cerium NPs, yield ions that are toxic for prokaryote cells (but well tolerated in eukaryote), acting as disinfectant and bacteriostatic agents. iv) Nanosafety: the prevention of unwanted effects produced by nanoparticles. This is one of our core-expertise areas and I have been working on it, developing NP models for toxicity and ecotoxicity testing, since FP06. My job is to understand which features of NPs poses health threats and then modify the NPs and the way they are used, to avoid the related hazards or exposure (risks) while maintaining the parental desired NP properties.


All this efforts have been translated into the creation of a spin-off company to exploit this knowledge: Nanotargeting (www.nanotargeeting.com), who is actually performing the regulatory preclinical studies and preparing the phase I clinical trials of Aurocis (cisplatin bounded AuNPs).



b. APPLICATION ON ENERGY.

We are facing energy and environmental threats that challenge our world: decrease/consumption of the fossil fuel reserves, global increase in energy demand, increasing pollution and the need to improve the processing of organic waste into a sustainable waste management, since damage to the environment is sooner or later translated into an energy cost. I am working on the design of advanced catalyst (multimetallic and heterodimer NPs) that are able to improve energy-chemical processes. Interestingly, energy harvesting and energy transfer processes are based on physic-chemical principles (electromagnetism) at the scale of few nm, from photosynthesis to electrical transport. I am also working on the design of a new generation of NPs for the production of hydrogen (with CdSe-Pt NPs) for reduction process and transform biomass and produce biofuels. Also I work on the use of iron oxide NPs to boost Biogas production thanks to the fact that iron ions are essential for the bacterial consortia responsible for the degradation and transformation of organic matter into methane. Our approach consists on the use of small concentrations of iron oxide nanoparticles designed and functionalized such that they progressively dispensing active iron ions at the necessary dose (not too low, not too high) for the bacteria, in analogy to sustained drug delivery, boosting methane production up to 300%. This work has been patent and it has received funds from the Bill and Melinda Gates foundation. This project has been granted the second SEGIB international prize and received support from the Programa Emprendedores of the REPSOL foundation leading to the creation of a Spin-Off, Applied Nanoparticles, dedicated to nanotechnology and energy/environment solutions which has, among other, the mission to exploit our Biogas enhanced production patent. I would like to stress that to me energy and environment are closely linked, while indeed, health and environment, are also extremely connected, since the health of the environment determine our morbidity. Regarding environment we address two issues, environmental toxicity of NPs and environmental remediation with NPs. I also focus on the electrochemical oxidation of molecules with our hollow Pt NPs where we are observing also important reactivity (and efficiency) boosts. Finally, I started in collaboration with the chemical engineering department of the UAB, with funding from the Fundación Ramón Areces, the study of nanostructures for the absorption of CO2, in such a way that in the future, the photocatalysis will reduce CO2 to other C species (CO, CH4, CH3OH, CHOOH) and it will be oxydized to recover energy and absorbed to avoid emissions and be transformed into a raw material in a close carbon cycle (note that 75% of south Africa gasoline is synthetic).


My work has position me as a privileged observer of the development of nanotechnology allowing me to advise and communicate to a broad audience. This is translated in participating as Project (e.g.: serenade-labex) or Industry (Nanonica) Scientific Advisor or communicating Science to Society, I am specially proud about the ebook “Nanoparticles before Nanotechnology” with more than 14.000 downloads. Also, the  reporting of my activities in international media, as the BBC or The Guardian also indicates the societal the impact of my work.

IP: Franco Puntes, Victor

Projectes

Nanopartícules farmacocinètiques

IP: Franco Puntes, Victor
Col·laboradors: Nanopartícules farmacocinètiques, Ramis Garcia, Joana, Nanopartícules farmacocinètiques, Montaña Ernst, Lena Nerea, Salazar Montoya, Vivian Angelica
Entitat finançadora: AGAUR no fer servir-correcte 4301-37
Finançament: 40000
Referència: 2021 SGR 00878
Durada: 01/01/2022 - 31/12/2024

Antioxidant and antiinflammatory therapy for the treatment of preeclampsia

IP: Franco Puntes, Victor
Col·laboradors: Ramis Garcia, Joana
Entitat finançadora: Generalitat de Catalunya - Departament de Salut
Finançament: 89693.23
Referència: SLT017/20/000182
Durada: 14/07/2021 - 31/12/2024

Terapia basada en células madre asistida con nanopartículas de Ce02: un enfoque nanofarmacéutico innovador para tratar enfermedades degenerativas de la retina.

IP: Duarri Piqué, Anna
Col·laboradors: García Arumí, José, Rosell Aluja, Jordi, Franco Puntes, Victor, Isla Magrané, Helena, Ramis Garcia, Joana
Entitat finançadora: Instituto de Salud Carlos III
Finançament: 174845
Referència: AC19/00080
Durada: 01/01/2020 - 30/06/2024

NEW ANTIOXIDANT STRATEGIES FOR THE MULTIMODAL TREATMENT OF PREECLAMPSIA (AFRODITA)

IP: Carreras Moratonas, Elena
Col·laboradors: Garcia Ruiz, Itziar, Bonacina , Erika, Ramis Garcia, Joana
Entitat finançadora: Instituto de Salud Carlos III
Finançament: 111320
Referència: PI20/00606
Durada: 01/01/2021 - 30/06/2025

Publicacions

The development of highly dense highly protected surfactant ionizable lipid RNA loaded nanoparticles.

PMID: 36923400
Revista: Frontiers in Immunology
Any: 2023
Referència: Front Immunol. 2023 Feb 27;14:1129296. doi: 10.3389/fimmu.2023.1129296. eCollection 2023.
Factor d'impacte:
Tipus de publicació: Revisió en revista internacional
Autors: Bastus, Neus G; Gonzalez-Rioja, Ramon; Puntes, Victor; Salazar, Vivian A et al.
DOI: 10.3389/fimmu.2023.1129296

Exploring the Long-Term Tissue Accumulation and Excretion of 3 nm Cerium Oxide Nanoparticles after Single Dose Administration.

PMID: 36979013
Revista: Antioxidants
Any: 2023
Referència: Antioxidants (Basel). 2023 Mar 21;12(3):765. doi: 10.3390/antiox12030765.
Factor d'impacte:
Tipus de publicació: Article en revista internacional
Autors: Bastus, Neus G; Casals, Eudald; Casals, Gregori; Ernst, Lena M; Fernandez-Varo, Guillermo; Gusta, Muriel F; Jimenez, Wladimiro; Mondragon, Laura; Puntes, Victor; Ramis, Joana et al.
DOI: 10.3390/antiox12030765

Asymmetrical Plasmon Distribution in Hybrid AuAg Hollow/Solid Coded Nanotubes.

PMID: 36985887
Revista: Nanomaterials
Any: 2023
Referència: Nanomaterials (Basel). 2023 Mar 9;13(6):992. doi: 10.3390/nano13060992.
Factor d'impacte:
Tipus de publicació: Article en revista internacional
Autors: Arbiol, Jordi; Arenal, Raul; Bastus, Neus G; Genc, Aziz; Patarroyo, Javier; Puntes, Victor; Sancho-Parramon, Jordi et al.
DOI: 10.3390/nano13060992

Variations in Biodistribution and Acute Response of Differently Shaped Titania Nanoparticles in Healthy Rodents.

PMID: 37049267
Revista: Nanomaterials
Any: 2023
Referència: Nanomaterials (Basel). 2023 Mar 25;13(7):1174. doi: 10.3390/nano13071174.
Factor d'impacte:
Tipus de publicació: Article en revista internacional
Autors: Bastus, Neus G; Bigini, Paolo; Chakraborty, Indranath; Cui, Daxiang; Masood, Atif; Morelli, Annalisa; Parak, Wolfgang J; Pelaz, Beatriz; Puntes, Victor; Salmona, Mario et al.
DOI: 10.3390/nano13071174

Pharmacokinetics of PEGylated Gold Nanoparticles: In Vitro-In Vivo Correlation.

PMID: 35159859
Revista: Nanomaterials
Any: 2022
Referència: Nanomaterials (Basel). 2022 Feb 1;12(3). pii: nano12030511. doi: 10.3390/nano12030511.
Factor d'impacte: 5.076
Tipus de publicació: Article en revista internacional
Autors: Manova, Alena; Bastus, Neus G; Moriones, Oscar H; Kohl, Yvonne; Dusinska, Maria; Runden-Pran, Elise; Puntes, Victor; Nelson, Andrew; Gabelova, Alena; Simon, Peter et al.
DOI: 10.3390/nano12030511

How Does Immunomodulatory Nanoceria Work? ROS and Immunometabolism.

PMID: 35401546
Revista: Frontiers in Immunology
Any: 2022
Referència: Front Immunol. 2022 Mar 17;13:750175. doi: 10.3389/fimmu.2022.750175. eCollection 2022.
Factor d'impacte: 7.561
Tipus de publicació: Article en revista internacional
Autors: Ernst, Lena M, Puntes, Victor et al.
DOI: 10.3389/fimmu.2022.750175

The Interactions between Nanoparticles and the Innate Immune System from a Nanotechnologist Perspective.

PMID: 34835755
Revista: Nanomaterials
Any: 2021
Referència: Nanomaterials (Basel). 2021 Nov 6;11(11). pii: nano11112991. doi: 10.3390/nano11112991.
Factor d'impacte: 5.076
Tipus de publicació: Revisió en revista internacional
Autors: Ernst, Lena M, Casals, Eudald, Italiani, Paola, Boraschi, Diana, Puntes, Victor et al.
DOI: 10.3390/nano11112991

Antibody cooperative adsorption onto AuNPs and its exploitation to force natural killer cells to kill HIV-infected T cells.

PMID: 34394703
Revista: Nano Today
Any: 2021
Referència: Nano Today. 2021 Feb;36. doi: 10.1016/j.nantod.2020.101056. Epub 2020 Dec 20.
Factor d'impacte: 20.722
Tipus de publicació: Article en revista internacional
Autors: Buzon, Maria J, Puntes, Victor, Genesca, Meritxell, Astorga-Gamaza, Antonio, Vitali, Michele, Borrajo, Mireya L, Jaime, Carlos, Bastus, Neus, Serra-Peinado, Carla, Luque-Ballesteros, Laura et al.
DOI: 10.1016/j.nantod.2020.101056

Simple spectroscopic determination of the hard protein corona composition in AuNPs: albumin at 75.

PMID: 32692793
Revista: Nanoscale
Any: 2020
Referència: Nanoscale. 2020 Aug 7;12(29):15832-15844. doi: 10.1039/d0nr02379e. Epub 2020 Jul 21.
Factor d'impacte: 6.895
Tipus de publicació: Article en revista internacional
Autors: Vitali, Michele, Casals, Eudald, Canals, Francesc, Colome, Nuria, Puntes, Victor et al.
DOI: 10.1039/d0nr02379e

Tesis

Gold Nanoparticles as Drug Delivery Agents: Detoxifying the Chemotherapeutic Drug Cisplatin

Doctorand: Joan Comenge Farré
Director/s: Victor Franco Puntes
Universitat: Universidad Autònoma de Barcelona
Any: 2013

Actualitat

Notícies

Professionals sanitaris i de recerca s'han reunit per unir sinergies i buscar solucions innovadores.

Ofertes de treball

Technical Support Pharmacokinetic Nanoparticles Group_20240073
Data d'inici:
22/02/2024
Data de finalització:
07/03/2024
Document: Descarrega
Postdoctoral Researcher - Design and pharmacokinetics of nanoparticles
Data d'inici:
23/02/2023
Data de finalització:
09/03/2023
Document: Descarrega
Predoctoral Researcher - Design and pharmacokinetics of nanoparticles
Data d'inici:
23/02/2023
Data de finalització:
09/03/2023
Document: Descarrega