This mouse model was generated by crossing Ppargc1a floxed mice with mice expressing Cre recombinase under the adipose-specific FABP4 promoter (Ap2-Cre mice). The PGC1alpha-FAT-KO mice have a specific deletion of the Ppargc1a gene in adipocytes, resulting in a knockout of the gene in white and brown adipose tissues. The model exhibits a moderate reduction in mitochodrial biogenesis in brown adipose tissues but do No exhibit any overt metabolic phenotype.

This mouse model was generated by crossing Ppargc1b floxed mice with mice expressing Cre recombinase under the adipose-specific FABP4 promoter (Ap2-Cre mice). This mouse model has a specific deletion of the Ppargc1b gene in adipocytes, resulting in a knockout of the PGC1beta gene in white and brown adipose tissues. The model exhibits a moderate to severe reduction in mitochodrial biogenesis in white and brown adipose tissues but do not exhibit any overt metabolic phenotype.

Model generated by moderate exercise routine

The left anterior descending coronary artery will be occluded with an intracoronary angioplasty balloon for 40-60 min. Then animals will be allowed to recover for 4 or 28 days. Infarct or scar size will be measured at the end of those periods. Cardiac function will be assessed by echocardiography.

The anterior coronary artery will be dissected at two different points. The most proximal will be located immediately below the first diagonal branch, and will be used to perform coronary occlusion, while the second will be located one centimeter distal to the first, and will be used to measure coronary blood flow

Partial and calibrated ligation of the portal vein causing hyperdynamic syndrome at 4 days and after a 2 weeks the systemic portal shunting is close to 100%.

This mouse model was generated by inserting loxP sites flanking exons 4-5 of the Ppargc1a (encoding for the co-activator PGC1alpha). The model enables time-restricted and tissue-specific ablation of the Ppargc1a gene.

This mouse model was generated by inserting loxP sites flanking exons 4-5 of the Ppargc1b (encoding for the co-activator PGC1beta). The model enables time-restricted and tissue-specific ablation of the Ppargc1b gene.

Primary tumor generated from cell lines.

The coronary artery will be identified and blocked using a 4/0 silk, placed 1 mm distal to the tip of the left atrium. All animals will undergo 60 minutes of coronary occlusion followed by 24 or 21 days of reperfusion. Function is assessed by echocardiography.