Tumor cells will be inoculated in the lateral tail vein.

Tumor induction by injection of different cell lines that have been derived from UV-induced melanoma models and patientd derived xenografts

The kidney transplant species will be wistar and the recipient Lewis, which are full MHC mismatch. This method provides us the natural immunological response of two genetically different species with a mixed transplant rejection of the organ.

Bone marrow transplantation is performed ussing either autologous or allogeneic bone marrow stem cells from either recipient C57BL/6 mice or donor Balb/c mice, respectively. Such cell transplantation is performed after a conditioning regimen based on either with or without total body irradiation and cyclofosfamide.

The heart transplant species will be Balb/c and the recipient C57BL/6, which are full MHC mismatch. This method provides us the natural immunological response of two genetically different species with a mixed transplant rejection of the organ.

Pre-sensitization is added to the heart transplant by an allogeneic skin transplant from the same donor. The heart transplant species will be Balb/c and the recipient C57BL/6. This method provides the specific immunological sensitization against the donor prior to transplantation to create immunological memory and increase the immune response against the organ.

Mice will be injected intraperitoneally with a single dose of LPS (dissolved in physiological saline), at 5 mg/Kg. Function is assessed by echocardiography

The coronary artery will be identified and blocked using a 6/0 silk, placed 1 mm distal to the tip of the left atrium. All animals will undergo 45 minutes of coronary occlusion followed by 3 or 28 days of reperfusion. Function is assessed by echocardiography

After two weeks of being fed with specific diets to generate obese rats, the glucose levels will be measured in all the animals, hoping that the diabetic animals present a plasmatic glycemia ≥ 200 mg/dl

Brain (U87-MG), lung (A549) and prostate cells (PC-3)Cells will be injected subcutaneously into the right flank of 6-8 week old female or male immunodeficient mice.