Pre-sensitization is added to the heart transplant by an allogeneic skin transplant from the same donor. The heart transplant species will be Balb/c and the recipient C57BL/6. This method provides the specific immunological sensitization against the donor prior to transplantation to create immunological memory and increase the immune response against the organ.

Mice will be injected intraperitoneally with a single dose of LPS (dissolved in physiological saline), at 5 mg/Kg. Function is assessed by echocardiography

The coronary artery will be identified and blocked using a 6/0 silk, placed 1 mm distal to the tip of the left atrium. All animals will undergo 45 minutes of coronary occlusion followed by 3 or 28 days of reperfusion. Function is assessed by echocardiography

After two weeks of being fed with specific diets to generate obese rats, the glucose levels will be measured in all the animals, hoping that the diabetic animals present a plasmatic glycemia ≥ 200 mg/dl

Brain (U87-MG), lung (A549) and prostate cells (PC-3)Cells will be injected subcutaneously into the right flank of 6-8 week old female or male immunodeficient mice.

Muscle injury due to micro-rupture of muscle fibers by surgical intervention Muscle lesion will be generated by performing a transverse biopsy, using a 6 mm diameter trocar, at the level of the myotendinous junction of the medial gastrocnemius muscle of the right hind leg

Fed for 12 or 20 weeks with a hypercaloric diet combined with a glucose-fructose drink (high fat glucose-fructose diet, HFGFD), in which the degree of non-alcoholic steatohepatitis (NASH) + fibrosis that they develop is evaluated Combination effect of 2 factors: Genetic + Dietetic

Fed for 8 weeks with a hypercaloric diet combined with a glucose-fructose drink (high fat glucose-fructose diet, HFGFD), in which the degree of non-alcoholic steatohepatitis (NASH) develop is evaluated

This mouse has a specific deletion of the Nr1f3 gene in adipocytes, resulting in a knockout of the gene in white and brown adipose tissues. So far, no pheNoype has been described.

Fetal surgery will be performed a complete laminectomy on three lumbar vertebrae on day 75th (+/- 5)